Seminested polymerase chain reaction and heteroduplex analysis
detects the monoclonality of IgH rearrangement in follicular lymphoma patients with high sensitivity.
Later on, more efficient methods have been discovered including heteroduplex analysis
(Sitte et al.
Quantitative heteroduplex analysis
for single nucleotide polymorphism genotyping.
Identification of mosquito avian-derived blood meals by polymerase chain reaction heteroduplex analysis
, a method discontinued in 2002, identified four additional mutations found with elevated frequency in African Americans.
The automated analysis used the heteroduplex analysis
plug-in for BioNumerics software (version 5.
Indeed, using heteroduplex analysis
and subsequently verifying the identified changes by DNA sequencing, the authors discovered APOE sequence variants in 3 of 765 patients, a frequency that was considerably higher than in nondiabetic patients.
Conventional mutation scanning methods require a separation step and include single-strand conformational polymorphism (SSCP) analysis (5), denaturing gradient gel electrophoresis (6), heteroduplex analysis
(7), denaturing HPLC (8), and temperature gradient capillary electrophoresis (9).
Comparison of single-strand conformation polymorphism and heteroduplex analysis
for detection of mutations in Charcot-Marie-Tooth type 1 disease and related peripheral neuropathies.
Earlier studies on heteroduplex analysis
suggested that the kinetics of heteroduplex formation would fit a quadratic model (26, 27).
This form of heteroduplex analysis
has focused on detection, and although it may involve a quantitative threshold, the result is essentially binary.
PCR AMPLIFICATION AND DENATURING HPLC HETERODUPLEX ANALYSIS