Fusion proteins were produced by use of modular cassettes containing signal sequence and restriction sites for cloning of variable and constant regions of both entire heterotetrameric antibodies as well as homodimeric
scFv-C,12-4 or scFv-C,12-3 (Fig.
glycoprotein produced by hepatocytes, is the major sex steroid-binding protein in plasma.
, fructose-binding lectin was isolated from Del Monte bananas by using a protocol that involved ion-exchange chromatography on DEAE-cellulose and SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75.
Endoglin (CD105) is a transmembrane homodimeric
glycoprotein produced mainly in endothelial cells, but is also present at lower concentrations in activated monocytes (8-10).
This value approximates the expected 25% enzyme activity in a homodimeric
enzyme (such as TP) with a single mutant allele that obliterates enzyme activity in heterodimeric (mutant plus wild-type) or homodimeric
sporamin-type trypsin inhibitor with antiproliferative, HIV reverse transcriptase-inhibitory and antifungal activities from wampee (Clausena lansium) seeds.
According to the manufacturer's information, this assay detects both the monomeric and homodimeric
forms of osteoprotegerin equally.
Notably, the homodimeric
form of recombinant OPG exerted more potent biologic activity than the monomeric form in vivo (5-7).
TfR, the homodimeric
membrane receptor (41), binds an iron-rich Tf molecule at the cell surface pH of 7.
VEGF is a 34- to 42-kDa heparin-binding, dimeric, disulfide-bound glycoprotein existing in at least five homodimeric
Gramicidin exists in a homodimeric
form wherein both monomers of the protein reside in a linear conformation.
Class I enzymes (a, [beta], and [gamma]) form homodimeric
and heterodimeric isoenzymes, the subunits of which are encoded by the ADH1, ADH2, and ADH3 genes, respectively, and play an important role in the metabolism of ingested ethanol.