Hurtley's test

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Hurtley's test [′hərt·lēz ‚test]

(pathology)

A test for the presence of acetoacetic acid in urine: take 10 cubic centimeters of a urine specimen and add 2.5 cm³of concentrated hydrochloric acid and 1 cm³of a freshly prepared 1% solution of sodium nitrite, then shake and let stand for 2 minutes; add 15 cm³of 0.880 ammonia and 5 cm³of 10% ferrous sulfate, then shake and pour into a large boiling tube, allowing it to stand; a violet or purple color confirms the presence of acetoacetic acid.

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