Now, we succeeded in producing IFN gamma
protein from their milk.
We have identified a new pathway, centered on IFN gamma, that controls the bone remodelling process both in-vivo and in-vitro.
We realised that this differentiation process involved IFN gamma-related genes, but also that these bone cells precursors could both be stimulated by IFN gamma and produced IFN gamma.
In the next step, the researchers focussed on an animal model where IFN gamma effect is blocked by inactivating its receptor-a model called IFN gamma receptor knock-out.
These findings confirm that IFN gamma is an integral factor for mesenchymal stem cells' differentiation into osteoblasts also in-vivo," said Kremer.
To everyone's surprise, the interferon tested, IFN gamma
, made MS attacks worse.
It will allow researchers to produce the IFN gamma
protein more abundantly and cheaply.
PBMCs were cultured with PHA as positive controls and all the Ii-Key hybrids as well as epitope-only controls for both IFN gamma analysis by BD ELISPOT and T cell proliferation measured by tritiated thymidine incorporation.
Both basal IFN gamma and proliferation were normal in patients with T1DM, and there was no significant difference between patients and controls.
Data also indicated induction significant NP-specific class I-restricted IFN gamma
10(11) viral particles); * clinical responses observed both locally and at distant sites, leading to an overall response rate of 60% (four complete and two partial responses out of ten evaluable patients); * gene transfer and expression of the IFN gamma
gene on both protein and messenger RNA levels; * up-regulation of the genes involved in immune response.
is a key cytokine involved in Th1 responses.