Immunofluorescence


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Immunofluorescence

A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See Immunoassay

Immunofluorescence

 

any set of methods of fluorescent analysis used in immunology, histochemistry, virology, bacteriology, mycology, and parasitology.

The combination of immunochemical reactions with fluorescence microscopy makes possible the detection of tissue and cellular antigens, including those involved in autoimmune diseases and in malignantly degenerated cells. The method is also useful in studying the patterns of antibody synthesis and in identifying the causative agents of many viral and microbial diseases. Specific antibodies are tagged with a fluorescent dye (for example, acridine orange) that will not alter their properties and then introduced to the specimen, so that only the parts of the specimen containing antigen will fluoresce. When the formation of antigen-antibody complexes is being investigated by immuno-fluorescence, the antibodies are tagged with a dye whose fluorescent properties will change when the antibodies combine with antigen.

REFERENCE

Immunofliurestsentsiia. Edited by J. Kubica. Moscow, 1967. (Translated from Polish.)

immunofluorescence

[¦im·yə·nō·flə′res·əns]
(immunology)
Fluorescence as the result of, or identifying, an immune response; a specifically stained antigen fluoresces in ultraviolet light and can thus be easily identified with a homologous antigen.
References in periodicals archive ?
The distinctive immunofluorescence pattern of TRITC-labeled PG-M3 staining in neoplastic promyelocytes was easily detected and interpreted.
Simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence.
Detection of anti-SSA antibodies by indirect immunofluorescence.
Direct immunofluorescence is performed with nasopharyngeal aspirates to detect viral antigen on pharyngeal columnar epithelial cells by staining them directly with fluorescent antibody.
Trask, Quantitative Immunofluorescence in Flow Cytometry, Proceedings of the VI International Conference on Immunofluorescence and Related Staining Techniques held in Vienna, Austria on April 6-8, 1978, W.
Pathology Diagnostics Ltd now has an immunofluorescence lab and is developing the IMF test portfolio to include a wider range of double immunohistochemistry (IHC) and immunofluorescence (IMF) assays.
In all cases, the diagnosis was confirmed by direct and/or indirect immunofluorescence studies.
This volume contains plenty of color photographs, enhanced by immunofluorescence, that vividly capture these microscopic biological processes in stunning detail.
All NPAs were examined for Pneumocystis jiroveci using immunofluorescence and polymerase chain reaction (PCR).

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