immunoprecipitation

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Related to Immunoprecipitated: Coimmunoprecipitation

immunoprecipitation

[‚im·yə·nō·prə‚sip·ə′tā·shən]
(immunology)
A protein purification method which involves the formation of an antibody-protein complex to separate out the protein of interest.
References in periodicals archive ?
1 showed that the HNK-1 carbohydrate was present on the antigen immunoprecipitated with MAB 1H12 (Fig.
Using this approach, we have correlated levels of CD117 immunoprecipitated from lysed endometrial tissue with immunostaining intensity, thereby confirming expression of CD117 in some malignant endometria.
After performing cell lysis and fractionation yielding Triton-soluble and Triton-insoluble fractions, we immunoprecipitated both fractions with an anti-FAK antibody.
The paper entitled "Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA" appeared in the online version of Nature Methods yesterday.
In accordance with these findings, atrazine did not enhance c-Src kinase activity on enolase as determined in BG-1 cell lysates immunoprecipitated with anti-c-Src antibody (data not shown).
5 mg of the lysate protein was immunoprecipitated using monoclonal antiserum of c-Met (1 [micro]g) and 15 [micro]1 of protein A/G-agarose (Santa Cruz).
Exudate fluids were immunoprecipitated with antimouse IgG Dynal[R] magnetic beads (Invitrogen) with, as capture antibodies, 5A6G7, 01G, MEM-G/9 or G233 anti-HLA-G antibodies, W6/32 anti-pan-HLA-I antibody, or an unspecific isotypic antibody.
The 43- and 40-kDa IGFBPs could be immunoprecipitated using a pIGFBP-3 antiserum, but not by a hIGFBP-3 antiserum (Figure 4).
HUT-78 cell lysates will be collected, Ikaros protein immunoprecipitated, and two-dimensional electrophoresis gels performed.
Customers can access NimbleGen ChIP-chip HD2 arrays by ordering arrays, instrumentation, and software and performing the research in their laboratory or core facility or by simply submitting immunoprecipitated and input samples to the Roche NimbleGen service facility for full service analysis.
Formalin-fixed chromatin was immunoprecipitated overnight with 10 [micro]L of polyclonal GR antibody (Cidlowski et al.
Immunoprecipitated DNA was analyzed by real-time PCR and compared to input DNA.