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immunoprecipitation
(redirected from Immunoprecipitated)

    Also found in: Medical, Wikipedia 0.01 sec.
immunoprecipitation [‚im·yə·nō·prə‚sip·ə′tā·shən]
(immunology)
A protein purification method which involves the formation of an antibody-protein complex to separate out the protein of interest.
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After lysing cells with cold TBS containing 1% TritonX-100 and protease inhibitor cocktail (Roche) followed by centrifugation, the supernatants were immunoprecipitated with anti-FLAG M2 antibody (Sigma).
NEIL2-Initiated, APE-Independent Repair of Oxidized Bases in DNA ... by Feridoun Karimi-Busheri / Biological sciences community
Immunoprecipitated DNA samples were then set to real-time PCR analysis to quantify the relative amount to their corresponding input controls with a LightCycler (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions.
Atrazine-induced aromatase expression is SF-1 dependent: implications ... by Fan, WuQiang; Yanase, Toshihiko; Morinaga, Hidetaka; Gondo, Shigeki; Okabe, Taijiro; Nomura, Masatos / Environmental Health Perspectives
Strips of 21-nucleotide-long DNA subsequently are released from the immunoprecipitated DNA to create "genomic signature tags," which subsequently are identified in the international genome database.
Pathway identification imperils disease by USA Today (Magazine)
 
 
 
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