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in situ hybridization
(redirected from In situ hybridisation)

   Also found in: Medical, Wikipedia 0.06 sec.
in situ hybridization [‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.


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Use of fluorescence in situ hybridisation (FISH) to assess effects of smoking, caffeine, and alcohol on aneuploidy load in sperm of healthy men.
 
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