isoelectric focusing

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isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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Quality control for ESBL detection, isoelectric focussing and PCR: K.
Six randomly selected isolates (2 each from TEM, SHV and TEM+SHV positive groups) were subjected to isoelectric focussing.
12,13) Alternatively, for techniques like capillary isoelectric focussing and capillary gel electrophoresis the electroosomotic flow is eliminated.
Isoelectric focussing of this sample revealed several bands migrating between pI ~6 and 8.
Separation and identification of alkaline phosphatase isoenzymes and isoforms in serum of healthy persons by isoelectric focussing.