isoelectric focusing

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isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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Analysis of the iron-binding sites of transferrin by isoelectric focussing.
This test involves separation of EPO idiotypes by isoelectric focussing, immunoblotting, and detection of IgG labeled with streptiverdin-peroxidase.
The different modes of capillary electrophoresis include capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic capillary chromatography (MECC), isoelectric focussing and isotachophoresis (ITP).
Coverage includes electrophoretic techniques such as isoelectric focussing, SDS page, 2-D page, DIGE, liquid-chromatography techniques such as ion exchange, affinity chromatography or reversed-phase HPLC, and mass-spectrometric techniques such as MALDI, ESI, or FT ICR.