Two 10 ml samples of the AP preparation were placed in 20 ml Kimax
centrifuge tubes and centrifuged in an IEC HNSII centrifuge (Damon/IEC Division, Needham HTS, Massachusetts, USA) at 3 000 rpm for 10 minutes.
For the derivatization reaction, an aliquot of 50 [micro]L of supernatant was removed and added to 16 Kimax
glass test tubes (125 mm; Kimble/Kontes, Vineland, NJ) containing 1.
Ail tubes are constructed of KIMAX
borosilicate glass with two grades available: KG-33 glass is the lowest thermal expansion glass tubing offering the highest degree of thermal shock resistance for high temperature applications; and N-51A is low thermal expansion glass tubing to accommodate ambient temperature applications.
The glassware, high performance, high quality KIMAX
borosilicate glass, is durable when properly cared for.