Test specificity for the L-1, L-2 or L-3 test is based on the pH of the test solution for which the corresponding isozyme has optimum activity.
Samples were left undisturbed for at least 10 min for the L-1 or the L-2 test or at least 30 min for the L-3 test.
4 [micro]L per sample for the L-3 test was added by using a pipette tip with the tip cut off.
The minimum amount of dye made for the L-3 test was for 20 samples to ensure pipetting accuracy.
The L-3 test was the slowest and the least stable of the three tests.
Evaluation of a bulk of seed chips did not provide satisfactory results for the L-3 test.