To detect '6S rRNA , mdh , phoE , gadA/B , lacZ
, uidA (, cadA and tnaA (Camilla et al.
Pathogenicity in STEC OH7:Kl:H7 is most likely multifactorial and results from a novel combination of lack of cadA and lacZ
expression and the presence of stx1 and the intimin-like sivH genes, demonstrating pathoadaptivity and horizontal gene transfer.
To extend these observations, we quantified the mRNA levels of lacZ
and endogenous atRA-target genes, including Crabp2 (cellular retinoic acid binding protein), Ram, and Rarg (Balmer and Blomhoff 2002).
Expression of lacZ
in both promastigotes and amastigotes could be clearly visualized by fluorescence microscopy or by light microscopy with 5-bromo-4-chloro-3-indolyl- [beta]-D-galactopyranoside (CPRG).
recombination deficient host Escherichia coli ProA2, leuB6, thi-1, lacY1, Sambrook et al.
The pUC19 vector consisted of resistance gene and lacZ
gene encoding [beta]-galactosidase, an enzyme that converts substrate such as X-Gal (5-bromo-4-chloro-3-indolyl-[beta]-D-galactopyranoside).
When termination occurs, the LacZ
gene is not expressed and the RNA produced terminates with the inserted DNA sequence.
Staining for lacZ
(B-galactosidase expression) showed typical collicular fusiform and stellate cells.
Three mutants: E70 (chemically induced), 1(3)02937 (a LacZ
insertion mutant), and caps 65.
18) In a study of 10 patients, catheter-mediated (Dispatch catheter, Boston Scientific, Natick, Mass) adenoviral delivery of LacZ
was performed in peripheral arteries in patients with chronic critical leg ischemia.
Polymerase Chain Reaction (PCR) methods have been developed, and lacZ
, lamB, and uid genes have been used to design primers for coliform detection .
The production of B-galactosidase by the lacZ
gene expressed from Pmer was measured at different external mercuric-ion concentrations.