Luciferases


Also found in: Dictionary, Medical.

Luciferases

 

enzymes that participate in bioluminescence.

Luciferases catalyze the aerobic oxidation of luciferins, the result of which is the conversion of the substrate to the electron-excited state and the release of the excess energy as light quanta. Living organisms store luciferases in photogenic cells or in specialized luminescence organs. Flavoprotein (molecular weight [mol wt], 85,000) is the luciferase in species of Achromobacter. Crayfish of the genus Cypridina contain an albumin (mol wt, 37,000-45,000), and beetles of the genus Photinus contain the luciferase euglobulin (approximate mol wt, 100,000). Even in organisms that contain the same luciferase, such as all species of fireflies, there are differences in luciferase structure and in the optimum conditions for its activity.

References in periodicals archive ?
Firefly luciferases are abundantly utilized as gene reporter and catalyze a bioluminescense reaction which are directly detected.
Application of the luciferases cell culture bioassay for the detection of refined petroleum products.
This novel, naturally-secreted Metridia luciferase was initially cloned by Bayer scientists for use in drug screening.
2004) Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa.
These creatures all owe their glow to an enzyme called luciferase.
For more than a decade, scientists have made use of luciferase or the genes that encode it.
Promega Corporation announces the launch of a novel luciferase that is smaller, brighter, and more versatile than any current bioluminescent enzyme.
NanoLuc(TM) technology consists of a new engineered luciferase and the Nano-Glo(TM) Reagent containing the furimazine substrate.
By linking various luciferase genes to other genes of interest spliced into cells, scientists can now measure the relative activity rates of multiple genes in cells over time.
We are pleased to include Promega's recombinant firefly and humanized Renilla luciferases as reporters in our product lines.
Expression in Escherichia coli of biotinylated luciferases using biotin acceptor peptides.
Luciferase is inactivated by the chemical reactions involved in direct labeling of antigens or antibodies (9).