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In this study, increase in the production of IL6, IL8, MMP3 and MMP1 was found in culture supernatant after stimulation by bacterial and viral products like peptidoglycan (TLR2 ligand), LPS (TLR4 ligand), PolyI:C (TLR3 ligand), flagellin (TLR5 ligand); however, CpG DNA (TLR9 ligand) and imiquimod (TLR7 ligand) had no effect on synovial fibroblasts.
Flagellin induced production of IL6, IL8, MMP1, and MMP3 after stimulation in FLS.
TLR 3 ligand polyI:C stimulated FLS to produce IL6, IL8, MMP3 and MMP1 but not TIMP1.
He further added, "The versatility of MMP1 and TYR, which are relevant for both cosmetic and therapeutic use, are a unique match with our sd-rxRNA platform, allowing us to advance different compounds in both areas of development.
MMP1 isa collagen-degrading enzyme that may play a role in tumorigenic cell metastasis and TGF-[beta] is a pleiotrophic growth factor with predominately antiproliferative qualities.
Recently, a naturally occurring sequence variation in the human MMP1 gene promoter was reported (10).
MMP1 mRNA expression in BT-483 and MDA-MB-231 had significantly decreased in curcumin treatment group compared with control group.
3 U of Taq DNA polymerase [AmpliTaq Gold (PE Biosystems) for MMP1, MMP13, PAI-1, and all microsatellite markers, and Taq DNA polymerase (Qiagen) for MMP3].
Gene Product (chromosome) Polymorphism size, (a) n (b) bp MMP1 (11) [G.
MMP1 and MMP3 polymorphisms in promoter regions and cancer.
3) recently investigated how insertion of a G nucleotide at--1607 by in the MMP1 promoter sequence affects regulation of the transcription of the gene.
To confirm the role of the MMP1 polymorphism in ovarian and other cancers and to investigate whether the 5A/6A polymorphism in the MMP3 promoter has any correlation with the progression of cancer, we determined frequencies of both alleles in 164 controls and 160 subjects with cancer (43 with breast cancer, 63 with colorectal cancer, 29 with pulmonary cancer, and 25 with ovarian cancer).
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