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In this study, four isozymes (esterase, alcohol dehydrogenase, malate dehydrogenase and acid phosphatase) and thirty SSR markers were employed for evaluating their application in purity identification of three-way cross QPM hybrid Shaktiman-1, single cross QPM hybrid Shaktiman-2 and their parental lines.
The absorbance at 340 nm of oxidation-reduction reactions catalyzed by malate dehydrogenase reduced over time.
IDH), malate dehydrogenase (MDH), malate dehydrogenase ([NADP.
The mix was incubated for approximately 5 min to stabilize chemical conditions in the reaction tube, and 60 units of malate dehydrogenase were then added to oxidize malate to oxaloacetate.
Of the enzyme systems initially assayed, alcohol dehydrogenase (ADH); lactate dehydrogenase (LDH); malate dehydrogenase (MDH); phosphohexose isomerase (PHI/PGI); and 6-phosphogluconate dehydrogenase (6-PGD) resolved consistently.
0); and lactate dehydrogenase (Ldh-1&2), glucose-6-phosphate isomerase (Gpi), and malate dehydrogenase (Mdh-1&2) on amine citrate (pH 6.
Another 400-[micro]L supernatant was used to measure succinate dehydrogenase (SDH), malate dehydrogenase (MDH), creatine kinase (CK) and lactate dehydrogenase (LDH) activities using commercial kits (Nanjing Jiangcheng Biochemical Institute, Nanjing, China).
The tested enzymes were aconitase (ACO), acid phosphatase (ACP), alcohol dehydrogenase (ADH), adenylate kinase (AK), arginine aminopeptidase (AMP), diaphorase (DIA), endopetidase (EP), esterase (EST), fructose-1,6-diphosphatase (FDP), glutamate dehydrogenase (GDH), glutamate oxaloacetate transaminase (GOT), hexokinase (HK), isocitrate dehydrogenase (ICD), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), malic enzyme (ME), peptidase with leucyl-alanine (PEP-LA), 6-phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (GPI), and shikimate dehydrogenase (SKDH) (Siciliano and Shaw, 1976; Vallejos, 1983; Van Geyt and Smed, 1984; Acquaah, 1992).
3-phosphoglycerol dehydrogenase and malate dehydrogenase (8).
A total of 16 enzyme systems were successfully assayed, namely, adenylate kinase, aldolase, esterase, fumarase, glutamate-oxalo-acetate transaminase, [Alpha]-glycerophosphate dehydrogenase, hexokinase, hydroxybutyrate dehydrogenase, isocitrate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, malic enzyme, phosphoglucomutase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, superoxide dimutase and triosephosphate isomerase (Saura et al.