The presence of 50 kDa mTcTXNPx and 46 kDa cTcTXNPx, both in the oxidized form, (8) is not observed in samples treated with mercaptoethanol
, (9) which likely prevents the formation of disulphide bonds and therefore prevents dimerization.
Then 20 ug protein were mixed with SDS-PAGE sample buffer and mercaptoethanol
to incubation at 100degC for 5 minutes, separated by 15% SDS-PAGE and transferred to PVDF membrane.
Under the reducing action of P mercaptoethanol
the homogenous 170 kDa band disappeared and few narrow bands with lower molecular weight were visible.
1M NaCl, 1mM EDTA, 1mM PMSF and 1mM AY- mercaptoethanol
2 M Beta mercaptoethanol
These ingredients together brought about mucolysis and acted as detergents.
HCl, petroleum ether, CaCO3 were purchased from Fluka Chemicals and chloroform, tris, KCl, mercaptoethanol
were purchased from Merck.
The antioxidants supplementation to the culture medium can be divided into two large groups: ones that are non-enzymatic or metabolic with low molecular weight, such as L-ascorbic acid (vitamin C), cysteine, alpha-tocopherol, and Beta- mercaptoethanol
and ones with an enzymatic action, such as superoxide dismutase, glutathione peroxidase and catalase (Nordberg and Arner,
Method 3 in combination with buffers 1 and 2, followed by LiCl and sodium acetate can remove the enlacing polysaccharides of nucleic acids for precipitation of RNA, while b- mercaptoethanol
helps to prevent oxidation reactions resulting in high integrity and amount of extracted RNA (Fig.
75, 2% SDS, 5% mercaptoethanol
, 10% glycerol and 0.
25 Table 2--Kappa index of enzyme-linked immunosorbent assay (ELISA-C) indirect ELISA (I-ELISA) and fluorescence polarization test (FPA) according to the gold standard based on the combination of complement fixation test and 2- mercaptoethanol
test for diagnosis of brucellosis in buffaloes.
In the presence of denaturing agents such as SDS and mercaptoethanol
the separation of components depends on their molecular masses.
These vitelline envelope proteins could be extracted by acetic acid, which yielded a higher amount of proteins than other chemical treatments, such as mercaptoethanol