5 ppm) into up to eight sharp resonances (signals from orthophosphate, [alpha]-glycerophosphate, (3-glycerophosphate, myo-inositol hexakisphosphate, RNA mononucleotides
and scy/fo-inositol hexakisphosphate) and one broad resonance (a signal from organic P in high-molecular-weight organic matter).
It is catalyzes the conversion at neutral pH of purine 5-prime mononucleotides
to nucleosides, the preferred substrate being AMP.
Cells that lose the protein product of this gene show a unique type of MSI profile: the affected repeats are larger (mostly tetranucleotides), and mononucleotides
are seldom involved.
As a consequence, there has been a move toward including more mononucleotides
and fewer dinucleotides in MSI testing panels.
Once inside, the microbe is minced and digested into amino acids, mononucleotides
, simple fatty acids and sugars.
Cladribine is a nucleoside analogue that becomes phosphorylated intracellularly to form active mononucleotides
Because the masses of the 4 mononucleotides
(dAMP, dTMP, dGMP, and dCMP) comprising DNA are known with great accuracy, highly precise measurements of mass can be used to derive a base composition (or a constrained list of base compositions).
Primers are generally not designed to (5) di/tri, tri/tetra or sequences containing stretches of more than five single nt, as these o/ten result in 1-bp alleles, (6) Highly imperfect loci, as these often contain single base insertions or deletions, also resulting in 1-bp alleles, and (7) Tetranucleotide repeats containing three mononucleotides
Orthophosphate monoesters include inositol phosphates, sugar phosphates, and mononucleotides
(Condron et al.
The repair enzymes that are affected are those enzymes that correct inaccurate copies of the DNA in areas of genes with multiple nucleotide repeats, usually mononucleotides
, dinucleotides, trinucleotides, or tetranucleotides.
The labeled mononucleotides
were separated on cellulose plates (F1440; Schleicher and Schull, Dassel, Germany) and revealed using the solvents as previously mentioned (15).
Colorimetric ELISA measurement of specific mRNA on immobilized-oligonucleotide-coated microtiter plates by reverse transcription with biotinylated mononucleotides