Phosphoglycerate It catalyzes the interconversion of 2-and 3- mutase
phosphoglycerate in the glycolytic and gluconeogenic pathways.
2_110 13 Cytidine deaminase HmC4778_234 14 Fasciclin domain-containing protein HmSNP4691_183 14 Heat shock protein 70 Hm3Dl0_1 15 Hemocyaninlike HmC4791_1099 15 Hypothetical protein HmC1449_847 16 Insulinlike growth factor binding protein 7 HmC18774_676 16 3-Hydroxy-3-methylglutaryl-CoA HmC22449_261 16 Protein disulfide isomerase HmC3835_411 16 Sorbitol dehydrogenase HmC45_3002 16 Hillarin HmC6061_1289 16 Phosphoglycerate mutase
2 HmC929_2563 17 Hexokinase Hm3B4_2 18A Ribosomal protein 1 Hm3B4_7 18A Ribosomal protein l HmC253_1545 18C PDZ and LIM domain protein Zasp Hmid2044 18C Hemocyanin Isoform 1 HmidILL2.
Methylmalonyl CoA mutase
) is the second and less central enzyme that requires vitamin B12.
In the isolates with ISCR2, the element was directly linked to a deleted version of a phosphoglucosamine mutase
gene, [DELTA]glmM, as has been reported on other occasions (Figure 2).
MMA are due to a deficiency of the adenosylcobalamin-dependent enzyme methylmalonyl-CoA mutase
, a defect in intracellular cobalamin metabolism, transcobalamin II deficiency, intrinsic factor deficiency, or dietary cobalamin deficiency, which is found in vegetarians.
The first structural efforts focused on chorismate mutase
from Bacillus subtilis (9).
Two genes were targeted for amplification, namely, a conserved region flanked by genus-specific primer binding sites in Helicobacter 16S rDNA and a species-specific sequence, glmM (ureC), encoding a phosphoglucosamine mutase
of H pylori.
Coli Ghorismate Mutase
Prephenate Dehydrogenase by Site-Directed Mutagenesis and Chemical Modification'.
Identification of Inhibitors of Cofactor-Independent Phosphoglycerate Mutase
(iPGM) for Potential Treatment of Lymphatic Filariasis, done in collaboration with New England Biolabs.
Spot Accession number number Protein name M17 K22E Keratin, type II cytoskeletal 2 epidermal M18 Q6NTA2 HNRNPL protein M19 Q32Q12 Nucleoside diphosphate kinase M20 ALDOA Fructose-bisphosphate aldolase A M21 PEBP1 Phosphatidylethanolamine-binding protein 1 M22 PGAM1 Phosphoglycerate mutase
1 N13 GMFB Glia maturation factor beta N14 HSPB1 Heat shock protein beta-1 N15 TP1S Triosephosphate isomerase M23 PGAM1 Phosphoglycerate mutase
1 M24 ENOA Alpha-enolase Spot Theoretical Theoretical Number of number Gene name Mr pl peptide Score M17 KRT2 65,678.
The researchers then sequenced those chorismate mutase
genes, compared the sequences, and identified unique regions in each sequence.
In case of spots which displayed reduced expression levels, structural proteins like Septin-2, cofilin-1 and macrophage capping protein; proteins involved in cellular processes like Annexin 1, Annexin 4, Annexin 5 and proteins involved in metabolism like phosphoglycerate mutase
, triosephosphate isomerase, peptidyl-prolyl cis-trans isomerase were identified (Figure 5; Table 2).