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Northern blotting

   Also found in: Medical, Wikipedia 0.01 sec.
Northern blotting [¦nȯrth·ern ′bläd·iŋ]
(genetics)
Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.


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The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting.
Compared with manual PCR quantification techniques, such as Northern blotting or RNase protection assays, real-time PCR offers an enormous time savings, greater sensitivity, superior precision, and a larger dynamic range.
 
 
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