osmium tetroxide

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osmium tetroxide

[′äz·mē·əm te′träk‚sīd]
(inorganic chemistry)
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1] HEPES buffer at room temperature for 2 h and then postfixed with 1% osmium tetraoxide in 30 mmol [l.
1991, "Mechanism of oxidation of reducing sugars by Osmium tetraoxide in alkaline medium by the stopped flow technique" Carbohydrate Research, 211, 235-243.
Samples were then washed in Sorenson's Phosphate Buffer (SPB) and stored in 1% osmium tetraoxide for two hours.
3 buffer solution), postfixed with osmium tetraoxide at 1% (v/v), and placed in dehydration slides with ethanol and amilum acetate, followed by a critical point desiccation process with C[O.
Briefly, glass or plastic coverslips containing the adherent bacteria were postfixed with 1% osmium tetraoxide, dehydrated by sequential ethanol concentrations, dried to critical point, and coated with a mixture of gold and paladium (27).
After being immersed in Karnovsky's fixative, tissue samples for transmission electron microscopy (TEM) were rinsed in 1% osmium tetraoxide, dehydrated by graded series of ethanol, and finally embedded in epon.
These samples were postfixed with 1% osmium tetraoxide, dehydrated with alcohol, included in plastic resin (EPON), cut with an ultramicrotome and contrasted with uranyl acetate and lead citrate.
The TEM analysis of the nanocomposites was done on the Hitachi H-800 after the specimen dyed by using osmium tetraoxide.
SEM preparation followed standard procedures, which did not include using osmium tetraoxide.
These samples were post-fixed with 1% osmium tetraoxide, dehydrated with alcohol, included in plastic resin (EPON), cut with an ultramicrotome and contrasted with uranyl acetate and lead citrate.
Animals were fixed in 6% glutaraldehyde, postfixed in 3% osmium tetraoxide, dehydrated, and embedded in Spurr' s medium as previously described (Trench et al.