5 mL of 20% (w/v) TCA and then made up to total volume of 10 mL with DW following incubation with 2 mL of 5 mM potassium ferricyanide
in an ice under the dark for 60 min.
Analytically pure sodium hydroxide (NaOH) and potassium ferricyanide
were supplied by Xiaoshan Chemistry Co.
To assess the stability of the thiochrome derivatives, controls and patients samples (n = 25) were assayed immediately after potassium ferricyanide
oxidation, then stored at 10[degrees]C in the dark and reassayed after 24 and 48 h.
0 g/%) were also oxidized by adding 1-3 mg of potassium ferricyanide
per ml of hemoglobin.
After cleaning and drying the cup and measuring spoons, mix 1 cup hot water with 2-1/4 tablespoons potassium ferricyanide
6) and potassium ferricyanide
(2 mL, were mixed, and then incubated at 50C for 20 min.
The chemicals (analytical grade) linoleic acid, DMPO (5, 5-dimethyl-1- pyrroline-Noxide), DPPH (1, 1- diphenyl-2 picrylhydrazyl), Ferrous Chloride, hydrogen peroxide, Ammonium Thiocyanate, Ferric Chloride, and potassium ferricyanide
The chemicals used were L-cysteine, D,L-homocysteine, L-methionine, adenosine (ADO), L-dithiothreitol, Triton X-100, pyridoxal 5-phosphate, and potassium ferricyanide
and were purchased from Sigma Chemical Co.
reagent was then injected intothe incubated reaction mixture stream which resulted in iron(II)-ferricyanide complex measured at 790 nm.
The following materials were obtained from Sigma-Aldrich: pyridoxal phosphate, pyridoxal, pyridoxamine, pyridoxine, dithiothreitol, sodium bisulfate, D,L-HCY, potassium ferricyanide
, and Triton X-100.
Tenders are invited for Potassium Ferricyanide
Keywords: Silver nanoparticle; Gemifloxacin mesylate; Luminol- potassium ferricyanide
system; Sequential injection analysis; Chemiluminescence