Fibroblast 3T3 cell line was purchased from (NCCS, Pune), Pune, india, poly (2-Hydroxy ethyl metha acrylate), sodium meta bisulphate, potassium persulphate
, silver sulfate, sodium borohydrate, PBS 10%, Dulbecco's Modified Eagle Medium (DMEM) containing serum was purchased from Sigma-Aldrich.
In the present research work, an effort has been made to chemically modify the human amnion by cross linking the protein with glutaraldehyde and later graft copolymerizing with poly hydroxyl ethyl methacrylate (PHEMA) using potassium persulphate
and sodium metabisulphate as initiators.
The surface of CHA was modified by grafting with GMA using potassium persulphate
and sodium meta bisulphite as initiators in aqueous medium at room temperature and subsequently coupled with gentamicin through epoxy groups.