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primer |
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primer1 an introductory text, such as a school textbook primer2 a substance, such as paint, applied to a surface as a base, sealer, etc. primer [′prīm·ər] (materials) A prefinishing coat applied to surfaces that are to be painted or otherwise finished. Also known as prime coat. (cell and molecular biology) A short ribonucleic acid (RNA) sequence that is complementary to a sequence of deoxyribonucleic acid (DNA) and has a 3′-OH terminus at which a DNA polymerase begins synthesis of a DNA chain. A short sequence of DNA that is complementary to a messenger RNA (mRNA) sequence and enables reverse transcriptase to begin copying the neighboring sequences of mRNA. A transfer RNA whose elongation starts RNA-directed DNA synthesis in retroviruses. (engineering) In general, a small, sensitive initial explosive train component which on being actuated initiates functioning of the explosive train, and will not reliably initiate high explosive charge; classified according to the method of initiation, for example, percussion primer, electric primer, or friction primer. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Amplicons were sequenced according to a primer walking strategy. De novo whole genome sequencing, re-sequencing, single tube sequencing, FDA submission-quality sequencing, publication-quality sequencing, GC-rich sequencing, RNAi template sequencing, serial analysis of gene expression sequencing, viral vector sequencing, 16S ribosomal RNA gene sequencing, primer walking, SNP and mutation analysis, and methylation analysis The AMPure(TM) PCR purification kit can be used to purify amplicons as small as 100 bp, producing a yield of 90 percent or greater; the purified products can be used in a variety of applications, including microarray, sequencing and primer walking. |
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