chromosome walking

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Related to Primer walking: Chromosome walking

chromosome walking

[′krō·mə‚sōm ‚wȯk·iŋ]
(genetics)
Sequential isolation of overlapping molecular clones in order to span large intervals on the chromosome.
References in periodicals archive ?
Our approach to DNA sequencing by primer walking bypasses the requirement for custom primer synthesis and relies on a universal set of 256 presynthesized oligonucleotide adaptors, referred to as indexers (17), and type IIS restriction endonucleases that cleave DNA at defined distances from their recognition sites, generating ambiguous 4-nucleotide (nt) 5' overhangs (18).
The AMPure(TM) PCR purification kit can be used to purify amplicons as small as 100 bp, producing a yield of 90 percent or greater; the purified products can be used in a variety of applications, including microarray, sequencing and primer walking.
Amplicons were directly sequenced by primer walking.