my recent work suggests that (i) developmental genes are often regulated by multiple enhancers, Sometimes located at great linear distances, (ii) the spatio-temporal activity of a large fraction of those enhancers remains unknown, (iii) enhancer-promoter interactions are usually established before the target gene is expressed and are largely stable during embryogenesis, And (iv) stable interactions seem to be associated with the presence of paused rna polymerase ii
at the promoter before gene activation.
The cap is added to cellular mRNA precursors made by RNA polymerase II
and to transcripts of viruses that replicate in the nucleus.
The MIT experiments showed that Npas4 binds to the activation sites of specific genes and directs an enzyme called RNA polymerase II
to start copying them.
Background: Molecular events that cause tumor formation upregulate a number of HOX genes, called switch genes, coding for RNA polymerase II
Some chapter topics include origins of specificity in protein-DNA recognition, transcript elongation by RNA polymerase II
, and adding new chemistries to the genetic code.
The Tat/TAR interaction recruits numerous cellular transcriptional coactivators to TAR, including P-TEFb (Positive Transcription Elongation Factor b), an RNA polymerase II
C-terminal kinase, resulting in the phosphorylation of the C-Terminal Domain (CTD) of RNA Polymerase II
Specifically, miRNAs originate in the nucleus as primiRNAs, precursors transcribed by RNA polymerase II
Accurate transcription initiation by RNA polymerase II
in a soluble extraction from isolated mammalian nuclei.
This event is catalyzed by DNA-dependent RNA polymerase II
Supporting that notion, Scully and his colleagues report in the May 27 Proceedings of the National Academy of Sciences that BRCA1 may be part of RNA polymerase II
holoenzyme, a large collection of proteins used to create strands of RNA, the first step in assembling a gene's protein.
The messenger rna (mrna) is synthesized by rna polymerase ii
transcribing a protein-coding gene.
Larschan and her colleagues measured how much of an enzyme called RNA polymerase II
was active in the X-chromosome.