We identified many different mutations in the POLR3A gene which codes for a key subunit of RNA Polymerase III
(Pol III), a highly conserved protein complex with a crucial role in gene expression, and many other important pathways," Dr.
The other transcription factors and RNA polymerase III
are presumed to be in excess over available TFIIIA, because tRNA genes are transcribed in the egg extract.
In addition to what was covered in an earlier patent, 7,294,504, issued to Allele Biotech last November, the current patent described more ways of using RNA polymerase III
and its promoters to express siRNA or shRNA in human or other mammalian systems.
The Nucleonics' HBV clinical candidate (NUC B1000) is an expressed interfering RNA (eiRNA) based product, consisting of a plasmid DNA construct that encodes four short interfering RNA (siRNA) molecules, each under the control of an RNA polymerase III