The maps of every isolate were obtained for restriction fragments
in the range of 100-250 kb and were used to assess strain similarities.
Using terminal restriction fragment
length polymorphism (TRFLP) analysis of PCR-amplified 16S-rDNA sequences, we characterized eubacterial community structure in soil collected on the Bloomington campus of Indiana University beneath native Canadian wildginger (Asarum canadense L.
Considering this fact, this study was undertaken to characterize the Indian isolates of Echinococcus granulosus to find out difference between Indian cattle, buffalo and sheep isolates on the basis of random amplification of polymorphic DNA (RAPD) PCR and PCR mediated restriction fragment
length polymorphism (PCR-RFLP) of internal transcribed spacer gene 1 (ITS1).
To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment
length polymorphsm analysis with Hhal and Sau961 in a double digest.
The GTT haplotype, constructed from the three adjacent restriction fragment
length polymorphisms was found to be over-represented among CP cases.
The RFMP genotyping assay is easily adaptable for the detection of other genetic variations, including polymorphisms, deletions, and additions, as well as motifs with multiple variations, because the range and sizes of the resulting restriction fragments
analyzed in MALDI-TOF MS can be chosen according to the investigator's needs.
Since the size of the restriction fragment
obtained was smaller than expected on the basis of cap b locus size, a possible explanation may be that the size of region 2 of cap e locus is smaller than that found in Hib strains.
We propose a simple scheme that minimizes the number of digests required and involves separation of restriction fragments
from the ND3/ND4 PCR product on an agarose-Synergel[TM] gel using only four restriction enzymes.
tuberculosis isolates obtained from different parts of India based on IS6110 element polymorphism using restriction fragment
length polymorphism (RFLP) analysis.
Several methods have been described for the detection of mutations in the structural region, such as PCR followed by restriction fragment
length polymorphism (RFLP) analysis (4).
IS6110-based restriction fragment
length polymorphism (RFLP) analysis on the DNA extracted from the isolates of 1,141 patients (77% of culture-positive patients) was performed as described previously (10).
were then separated on Metaphor FMC agarose gels (3% for Mse I digests of ITS1 products and 3.