length polymorphism analysis of 16S ribosomal DNA of Streptococcus and Enterococcus species of bovine origin.
The selected and interpolated traces were analyzed with custom software to identify restriction fragment
maps for the sample.
Resulting terminal restriction fragment
(TRF) profiles were standardized for analysis using procedures similar to those described by Dunbar et al.
based on mitochondrial DNA restriction fragment
Abbreviations: BC, backcross: CAPS, cleaved amplified polymorphic sequence; CS, Chinese Spring: HRS, hard red spring: MAS, marker assisted selection: PCR, polymerase chain reaction: RFLP, restriction fragment
Similar difficulties might confound the interpretation of restriction fragments
from the nuclear 28S ribosomal RNA PCR products.
Sizes of individual restriction fragments
from DNA chromosome of the Pseudomonas syringae pv.
granulosus on the basis of random amplification of polymorphic DNA-PCR (RAPD-PCR) and polymerase chain reaction based restriction fragment
length polymorphism (PCR-RFLP) to identify internal transcribed spacer genel (ITS1) variant.
The RFMP genotyping assay is easily adaptable for the detection of other genetic variations, including polymorphisms, deletions, and additions, as well as motifs with multiple variations, because the range and sizes of the resulting restriction fragments
analyzed in MALDI-TOF MS can be chosen according to the investigator's needs.
These two accessions, most likely, have a restriction site for this endonuclease at 380 bp and had two additional restriction fragments
length polymorphism analysis of Cryptococcus neoformans isolates from environmental (pigeons excreta) and clinical sources in New York City.
3) Does intraspecific variability compromise the use of mtDNA restriction fragments
in species identification?