of RNA viruses; applications and perspectives.
elegans as an in vivo model and apply large-scale forward and reverse genetic
screenings with high-throughput.
After general overviews, the sections cover population genetics and evolutionary approaches, forward and reverse genetic
systems for defining virulence, and comparative "omics" approaches to defining virulence.
Using reverse genetic
engineering, scientists have discovered that the 1918 influenza pandemic that killed fifty million people worldwide was a form of this virus.
Researchers' interests in gene deletion studies are fueled by the promise of gaining insight into the function of developmentally important genes discovered through the Genome Sequencing Projects and high-throughput reverse genetic
To this end the researchers performed a reverse genetic
loss-of-function screen with a small interfering RNA (siRNA) library representing 160 kinases.
By integrating diverse genomic-scale analyses and using reverse genetic
, we identify novel DG proteins that are singularly exported beyond the tachyzoites-hosting PV to the host cell nucleus, thus extending the scope of the function of DG proteins beyond their dedicated role in vacuole formation.
Specific topics encompass the paleobotany of Livingston Island, Antarctica; targeted isolation and sequence assembly and characterization of the white spruce; the complete chloroplast genome sequence of a tree fern; reverse genetic
analyses of gene function in fern gametophytes; glossopterid seed ferns from the Late Permian period; pollen development; DNA barcoding in the Cycadales; expressed sequence tag analysis in Ginkgo biloba; frequent fires in ancient shrub tundra; the history of native plant communities in the South; past vegetation patterns of New Mexico's Rio Del Oso Valley; and East Asian monsoon and paleoclimatic data analysis.
studies under way in our laboratory are designed to assess this possibility.
This highly infectious H5N1 strain is reassortanted with a human influenza virus strain through reverse genetic
technology; which is used to imitate the potential pandemic strain.
Now Yoshihiro Kawaoka and his colleagues at the University of Wisconsin-Madison School of Veterinary Medicine and the University of Tokyo have perfected an advanced method of producing the inactivated "seed" virus used to produce influenza vaccine, a technique known as reverse genetics
This process, a type of reverse genetics
, revealed a single mutation that rendered the virus virulent.