reverse genetics

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reverse genetics

[ri¦vərs jə′ned·iks]
(cell and molecular biology)
An experimental method in which information from cloned deoxyribonucleic acid (DNA) or protein sequences is used to find or to produce mutations that help identify the function of a gene or protein (in contrast to classical genetics in which a known function or trait is traced back to a particular gene.
References in periodicals archive ?
Functional characterization of bovine viral diarrhea virus nonstructural protein 5A by reverse genetic analysis and live cell imaging.
The difference between forward and reverse genetic approaches is the order of studying genotype and phenotype.
FMD virus serotype O vaccine strain with enhanced thermo- stability was constructed using reverse genetic approach, he added.
Objective: ExpoSEED aims to integrate forward and reverse genetic approaches to dissect the molecular mechanisms that control seed/kernel (hereafter referred as seed) yield in model species and to transfer the acquired knowledge to selected crops as legumes and cereals.
Scientists from Europe and the US discuss coronavirus and rhabdovirus reverse genetics, reverse genetic tools to study the hepatitis C virus, calcivirus genetics, modification of the measles virus and application to pathogenesis studies, bunyavirus reverse genetics and applications to studying interactions with host cells, using reverse genetics to improve influenza vaccines, Bluetongue virus reverse genetics, genetic modification in mammalian orthoreoviruses, and reverse genetics and quasispecies.
Unlike model organisms with large collections of mutant strains to draw upon, the lack of reverse genetic tools in the mosquito has made it is very difficult to assign functions to genes in a definitive manner," Adelman said.
Several reverse genetic strategies have been successfully used for functional genomics in animal and plant species including chemical mutagenesis (3).
cells destroyed by Alzheimer's disease, reverse genetic defects such as cystic fibrosis, and re-grow severed spinal cords.
The editors (both of the Centre for Crop Genetic Improvement, Rothamsted Research, UK) believe that the production and characterization of transgenic plants is a powerful reverse genetic strategy that can be used in cereals research to ascribe function to defined DNA sequences.
The upshot of all this, apparently, is that Brian Boru is "tail-male and tail-female to near reverse genetic relatives".
1981), and targeted gene disruption (Rong and Golic 2000) have allowed researchers to conduct molecular and reverse genetic analyses.
Now, using what is called a reverse genetic approach, he and his colleagues have been able to demonstrate the protein is exactly what they thought.