reverse genetics

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reverse genetics

[ri¦vərs jə′ned·iks]
(cell and molecular biology)
An experimental method in which information from cloned deoxyribonucleic acid (DNA) or protein sequences is used to find or to produce mutations that help identify the function of a gene or protein (in contrast to classical genetics in which a known function or trait is traced back to a particular gene.
References in periodicals archive ?
Reverse genetics of RNA viruses; applications and perspectives.
elegans as an in vivo model and apply large-scale forward and reverse genetic screenings with high-throughput.
After general overviews, the sections cover population genetics and evolutionary approaches, forward and reverse genetic systems for defining virulence, and comparative "omics" approaches to defining virulence.
Using reverse genetic engineering, scientists have discovered that the 1918 influenza pandemic that killed fifty million people worldwide was a form of this virus.
Researchers' interests in gene deletion studies are fueled by the promise of gaining insight into the function of developmentally important genes discovered through the Genome Sequencing Projects and high-throughput reverse genetic screens.
To this end the researchers performed a reverse genetic loss-of-function screen with a small interfering RNA (siRNA) library representing 160 kinases.
By integrating diverse genomic-scale analyses and using reverse genetic, we identify novel DG proteins that are singularly exported beyond the tachyzoites-hosting PV to the host cell nucleus, thus extending the scope of the function of DG proteins beyond their dedicated role in vacuole formation.
Specific topics encompass the paleobotany of Livingston Island, Antarctica; targeted isolation and sequence assembly and characterization of the white spruce; the complete chloroplast genome sequence of a tree fern; reverse genetic analyses of gene function in fern gametophytes; glossopterid seed ferns from the Late Permian period; pollen development; DNA barcoding in the Cycadales; expressed sequence tag analysis in Ginkgo biloba; frequent fires in ancient shrub tundra; the history of native plant communities in the South; past vegetation patterns of New Mexico's Rio Del Oso Valley; and East Asian monsoon and paleoclimatic data analysis.
Reverse genetic studies under way in our laboratory are designed to assess this possibility.
This highly infectious H5N1 strain is reassortanted with a human influenza virus strain through reverse genetic technology; which is used to imitate the potential pandemic strain.
Now Yoshihiro Kawaoka and his colleagues at the University of Wisconsin-Madison School of Veterinary Medicine and the University of Tokyo have perfected an advanced method of producing the inactivated "seed" virus used to produce influenza vaccine, a technique known as reverse genetics.
This process, a type of reverse genetics, revealed a single mutation that rendered the virus virulent.