Reverse transcribed definition of Reverse transcribed in the Free Online Encyclopedia.

reverse transcriptase [ri′vərs tran′skrip‚tās]

(genetics)

A polymerase that mediates deoxyribonucleic acid synthesis by using a ribonucleic acid template.

Reverse transcriptase

Any of the deoxyribonucleic acid (DNA) polymerases present in particles of retroviruses which are able to carry out DNA synthesis using an RNA template. This reaction is called reverse transcription since it is the opposite of the usual transcription reaction, which involves RNA synthesis using a DNA template. See Retrovirus

The transfer of genetic information from RNA to DNA in retrovirus replication was proposed in 1964 by H. M. Temin in the DNA provirus hypothesis for the replication of Rous sarcoma virus, an avian retrovirus which causes tumors in chickens and transformation of cells in culture, and reverse transcriptase has since been purified from virions of many retroviruses. The avian, murine, and human retrovirus DNA polymerases have been extensively studied.

Studies indicate that reverse transcriptase is widely distributed in living organisms and that all reverse transcriptases are evolutionarily related. For example, the organization of the nucleotide sequence of integrated retroviral DNA has a remarkable resemblance to the structure of bacterial transposable elements, in particular, transposons.

Reverse transcriptase genes are present in the eukaryotic organisms in retrotransposons and in retroposons or long interspersed (LINE) elements. Both of these types of elements can transpose in cells. See Deoxyribonucleic acid (DNA), Ribonucleic acid (RNA), Transposons

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No references found	In brief, viral RNA was extracted from 150 [micro]L of 10%-20% fecal suspensions in phosphate-buffered saline by using a guanidine and silica method (12) and reverse transcribed by using random hexamers (Sigma-Genosys, Dorset, UK). Human astrovirus gastroenteritis in children, Madagascar, 2004-2005 by Papaventsis, Dimitrios C.; Dove, Winifred; Cunliffe, Nigel A.; Nakagomi, Osamu; Combe, Patrice; Gros / Emerging Infectious Diseases The mRNA for these enzymes was assessed by quantitative reverse transcribed PCR in non-irradiated cells to establish a baseline level. Differential regulation of hyaluronan metabolism in the epidermal and ... by Knaggs, H.E. / Clinical Dermatology RNA from individual AP or PP was reverse transcribed and the DNA amplified and sequenced. Sperm Protein SP22 in somatotropes and thyrotropes of the rat and ... by Benoit, Allison; Blake, Charles; McCoy, George / Bulletin of the South Carolina Academy of Science	reverse engineer reverse engineering reverse fault reverse feedback reverse flange reverse genetics reverse graft reverse key reverse lay reverse mutation reverse osmosis reverse passive anaphylaxis reverse pinocytosis reverse pitch reverse polarity reverse Polish notation reverse polish syntax reverse power reverse print reverse process reverse proxy reverse saddle reverse similar fold reverse slip fault reverse slope Reverse transcribed reverse transcript reverse transcriptase reverse video reverse voltage reverse-acting diaphragm valve reverse-acting thermostat reverse-blocking tetrode thyristor reverse-blocking triode thyristor Reverse-Current Braking reverse-current cleaning reverse-current protection reverse-current relay reverse-direction flow reverse-flight stair reverse-flowage fold reverse-printout typewriter reverse-roll coating reverse-swing door reversed reversed air-blast process reversed arc reversed consequent stream reversed door reversed image reversed loader	Reverse Stockholm Syndrome Reverse subsidiary merger Reverse Supplemental Channel Reverse Supplemental Channel Type Reverse Supplemental Channel, Channel Element Reverse Supplemental Code Channel Type Reverse Supply Chain Management reverse swap Reverse Swaps Reverse T3 Reverse T3 Reverse Take-Over Reverse takeover Reverse takeover reverse takeovers reverse takeovers Reverse tarot meaning Reverse tarot meaning Reverse taxation reverse telecine Reverse telnet Reverse Tetracycline Transactivator Reverse Tetracycline-Regulated Retroviral Vector Reverse Tool Order reverse torque test reverse torque test reverse torque test reverse torque test reverse torque test Reverse Traffic Channel Reverse transcribed Reverse transcribing Reverse transcribing Reverse transcribing reverse transcript reverse transcriptase reverse transcriptase reverse transcriptase reverse transcriptase reverse transcriptase Reverse Transcriptase and Polymerase Chain Reaction Reverse Transcriptase In Situ Polymerase Chain Reaction reverse transcriptase inhibitor reverse transcriptase inhibitor reverse transcriptase inhibitor reverse transcriptase inhibitor reverse transcriptase inhibitor Reverse transcriptase inhibitors Reverse transcriptase inhibitors Reverse Transcriptase Nucleoside Inhibitor Reverse Transcriptase Polymerase Chain Reaction Reverse Transcriptase Positive Reverse Transcriptase Primer Extension Reverse Transcriptase Recognition Signal Reverse Transcriptase-Polymerase Chain Reaction Reverse Transcriptase-Polymerase Chain Reaction Method Reverse Transcriptase-Polymerase Chain Reaction Technique reverse transcription reverse transcription reverse transcription reverse transcription

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In brief, viral RNA was extracted from 150 [micro]L of 10%-20% fecal suspensions in phosphate-buffered saline by using a guanidine and silica method (12) and reverse transcribed by using random hexamers (Sigma-Genosys, Dorset, UK).

Human astrovirus gastroenteritis in children, Madagascar, 2004-2005 by Papaventsis, Dimitrios C.; Dove, Winifred; Cunliffe, Nigel A.; Nakagomi, Osamu; Combe, Patrice; Gros / Emerging Infectious Diseases

The mRNA for these enzymes was assessed by quantitative reverse transcribed PCR in non-irradiated cells to establish a baseline level.

Differential regulation of hyaluronan metabolism in the epidermal and ... by Knaggs, H.E. / Clinical Dermatology

RNA from individual AP or PP was reverse transcribed and the DNA amplified and sequenced.

Sperm Protein SP22 in somatotropes and thyrotropes of the rat and ... by Benoit, Allison; Blake, Charles; McCoy, George / Bulletin of the South Carolina Academy of Science