Also found in: Dictionary, Thesaurus, Medical, Wikipedia.
Related to Ribosomes: endoplasmic reticulum


Small particles, present in large numbers in every living cell, whose function is to convert stored genetic information into protein molecules. In this synthesis process, a molecule of messenger ribonucleic acid (mRNA) is fed through the ribosome, and each successive trinucleotide codon on the messenger is recognized by complementary base-pairing to the anticodon of an appropriate transfer RNA (tRNA) molecule, which is in turn covalently bound to a specific amino acid. The successive amino acids become linked together on the ribosome, forming a polypeptide chain whose amino acid sequence has thus been determined by the nucleic acid sequence of the mRNA. The polypeptide is subsequently folded into an active protein molecule. Ribosomes are themselves complex arrays of protein and RNA molecules, and their fundamental importance in molecular biology has prompted a vast amount of research, with a view to finding out how these particles function at the molecular level. See Genetic code, Protein, Ribonucleic acid (RNA)

Ribosomes are composed of two subunits, one approximately twice the size of the other. In the bacterium Escherichia coli, whose ribosomes have been the most extensively studied, the smaller subunit (30S) contains 21 proteins and a single 16S RNA molecule. The larger (50S) subunit contains 32 proteins, and two RNA molecules (23S and 5S). The overall mass ratio of RNA to protein is about 2:1. Cations, in particular magnesium and polyamines, play an important role in maintaining the integrity of the ribosomal structures. The ribosomes are considerably larger in the cytoplasm of higher organisms (eukaryotes). Nevertheless, all ribosomal RNA molecules have a central core of conserved structure, which presumably reflects the universality of the ribosomal function. See Cell (biology), Organic evolution

The process of protein biosynthesis is essentially very similar in both prokaryotes and eukaryotes; what follows is a brief summary of what happens in E. coli. The first step is that an initiator tRNA molecule attached to the amino acid N-formyl methionine recognizes its appropriate codon on a mRNA molecule, and binds with the mRNA to the 30S subunit. A 50S subunit then joins the complex, forming a complete 70S ribosome. A number of proteins (initiation factors, which are not ribosomal proteins) are also involved in the process. At this stage, the initiator aminoacyl tRNA occupies one binding site, the P-site (peptidyl site), on the ribosome, while a second tRNA binding site (the A-site, or aminoacyl site) is free to accept the next aminoacyl tRNA molecule. In the subsequent steps, the elongation process aminoacyl tRNA molecules are brought to the A-site as ternary complexes together with guanosine triphosphate (GTP) and a protein factor (elongation factor Tu). Once an aminoacyl tRNA is in the A-site, the initiator amino acid (or at later stages the growing polypeptide chain) is transferred from the P-site tRNA to the A-site aminoacyl tRNA. It is not clear whether this peptidyl transferase activity requires the active participation of a ribosomal component. After peptide transfer has taken place, the peptide is attached to the A-site tRNA, and an “empty” tRNA molecule is at the P-site. The peptidyl tRNA complex must now be translocated to the P-site, in order to free the A-site for the next incoming tRNA molecule. Here again protein factors and GTP are involved, and it is probable that the empty tRNA occupies a third ribosomal site (exit or E-site) before finally leaving the complex. Studies show that elongation factor G hydrolyzes GTP to bring about translocation of the tRNA molecules. The protein chain is completed by the appearance of a “stop” codon in the mRNA. This is recognized by yet another set of protein factors, which causes the completed polypeptide chain to be released from the ribosome. At any one time a number of ribosomes are engaged in the reading of a single mRNA molecule, which leads to the appearance of polyribosomes (polysomes). See Molecular biology

References in periodicals archive ?
Technological advances also give researchers an atomic-level, 3-D model of spectinomycin bound to bacterial ribosomes.
According to this new study, before recycling is complete, the protein Rqc2 can prompt the ribosome for the addition of two of the twenty total amino acids such as alanine and threonine, in any order.
5) Long regarded as simply tiny protein-manufacturing plants, ribosomes are now emerging as essential in the maintenance of normal cellular functions, and as key players in the science of "systemic aging" and disorders such as chronic fatigue syndrome.
Keywords: Escherichia coli, outer membrane, cytoplasmic membrane, periplasmic space, nucleoid, ribosomes, vesicles, aberrant mesosomes, betaine
In the cancer-promoting variants, a highly knotted region at the start of the strand must be unwound before a ribosome can bind and begin translating the genetic code into the amino acids that build our cellular proteins.
I was not able to locate an interactive, artistic activity in which students simulate translation; therefore, I devised an activity in which students visit the different parts of a ribosome, perform the steps that build a polypeptide chain, and construct a fully functional protein.
Additionally, Schimmer believes it's possible that chemists could design tigecycline analogues that would be more potent or more selective of mitochondrial ribosomes over bacterial ones.
Ribosomes produce proteins, which in turn control the chemistry of all living organisms.
They present 26 papers exploring these two primordial and present-day RNA Worlds through discussions of the role of RNA in the first life forms and the appearance of cells, characteristics of riboswitches and ribozomes, protein synthesis on ribosomes, recent discoveries concerning RNA regulated gene expression, and tools and methodologies for RNA science.
Their work revealed what ribosomes, which produce proteins that control the chemistry in all living organisms, look like and how they function at the atomic level.