SDS polyacrylamide gel electrophoresis

SDS polyacrylamide gel electrophoresis

[¦es¦dē¦es ‚päl·ē·ə¦kril·ə·mīd ‚jel i‚lek·trō·fə′rē·səs]
(cell and molecular biology)
An electrophoretic technique in which proteins are denatured by the negatively charged detergent sodium dodecyl sulfate, which masks their intrinsic electrical charge so that when the constituent polypeptide chains are run through a polyacrylamide gel, the protein molecules are separated according to size, not electrical charge. Abbreviated SDS-PAGE.
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This protein was found to be present in numerous cell cultures so I purified to homogeneity by preparative isoelectric focusing and SDS polyacrylamide gel electrophoresis this protein from human embryonic kidney cells (i.
The SDS polyacrylamide gel electrophoresis (PAGE)[14] method was used for the determination of complexation concentration of beta-cyclodentrin ([beta]-CD) with insulin.
Using the integrated LabChip(R) format, researchers will be able to replace the lengthy and laborious process required with traditional SDS polyacrylamide gel electrophoresis with a fast, automated and integrated system, designed to achieve high level resolution to support highly precise purity analysis.