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281][gamma] was located on SDS-PAGE by size, intensity of the band after IPTC induction, and confirmation that the band contained 6 x His by Western blot. We purchased protease inhibitor cocktail from Calbiochem (San Diego, CA), acrylamide from Boehringer Mannheim (Indianapolis, IN), and SDS-PAGE molecular weight standards and Western blotting supplies from Bio-Rad (Hercules, CA). coli, mammalian and insect systems (including Baculovirus expression), S35 labeling, purification of proteins using various affinity tags, SDS-PAGE, Western Blots, Lumio labeling and fluorescence detection methods |
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