Cell culture: HEK 293T Cells, the bovine muscle satellite cells
were given by NBCIC (National Beef Cattle Improvement Center, Northwest AandF University, Yangling, China), and identified with cell markers by Immunofluoresence staining, cultured in Dulbecco's modified Eagle's medium (DMEM, gibco) with stable L-glutamine supplemented with 20% fetal bovine serum (FBS, gibco), 10% horse serum (HS, gibco), 100U/ml penicillin (sigma), 100ug/ml streptomycin (sigma) (complete growth medium), incubated at 37 AdegC under 5% CO2 atmosphere (Thermo).
Culture and identification procedures of porcine satellite cells
The initial phase of activation and proliferation of satellite cells
is characterized by MyoD expression, which increases at the initial stage of the regeneration process and reaches its maximum three days after injury (Tidball and Villalta, 2010).
Miyata, "Muscle satellite cells
are activated after exercise to exhaustion in Thoroughbred horses," Equine Veterinary Journal, vol.
Briefly, monoclonal anti-Pax7 antibody (Developmental Studies Hybridoma Bank, Iowa, IA, USA) was used for the detection of muscle satellite cells
(Asakura et al.
When the researchers injected old mice with GDF11, the population of satellite cells
increased, and the number of satellite cells
with severe DNA damage fell by a factor of 4.
Although it has been noted previously that the fiber-type distribution of satellite cells
may differ between slow and fast limb muscles , this was not a primary aim of this study.
Changes in satellite cells
in human skeletal muscle after a single bout of high intensity exercise.
Muscle growth in fish depends on the proliferation and differentiation of myogenic precursor cells (termed satellite cells
or myoblasts) (JOHNSTON et al.
Significant role of satellite cells
(SC) in muscle regeneration following injury is well documented (19).
Now, researchers at the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), part of the National Institutes of Health, are beginning to find answers in a specialized population of cells called satellite cells
Previous research has demonstrated that with exercise, muscle cells get even bigger by merging with stem cells called satellite cells
, which are nestled between muscle fiber cells.