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Single-Step Reaction

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Single-Step Reaction 

a chemical reaction that cannot be represented as the sum of simpler chemical reactions. Stepwise chemical reactions (seeREACTION, STEPWISE CHEMICAL) consist of two or more single-step reactions, which are often referred to as simply “steps” of a complex reaction. As a rule, no more than two atomic bonds are broken or formed in a single-step reaction. For example, in the reaction H2 + O = H + OH, one H—H bond is broken, and one O—H bond is formed.



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A model which includes two parallel single-step reactions is used in this study.
Unincorporated dNTPs and excess primers were inactivated and degraded in a single-step reaction by the addition of 5 U of exonuclease I and 0.
 
 
 
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