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We reasoned that any gains in activity from the increased concentration of substrate and lower DBS extraction volume are more than likely offset by reduced extraction efficiency of GLA due to the lack of sodium taurocholate in the extraction buffer in the microfluidic protocol.
To the residue we added 15 [micro]L of 120 g/L sodium taurocholate in water.
We then added 240 [micro]L of an aqueous solution of sodium taurocholate (120 g/L) and oleic acid (12 g/L) to the vial, vortex-mixed the sample until the residue dissolved, and then added 2.