Southern blotting

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Southern blotting [′sə·th·ərn ‚bläd·iŋ]

(cell and molecular biology)

A technique for the detection of specific sequences among deoxyribonucleic acid (DNA) fragments whereby the fragments are separated by gel electrophoresis and then blotted onto a sheet of nitrocellulose for detection with radioactively labeled nucleic acid probes. It was first described by E. M. Southern in 1975.

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No references found			When the familiar broad smear of restricted DNA is visible after 50-100 volt-hours, full-scale electrophoresis for Southern blotting may commence. QA initiatives for the DNA lab by Farkas, Daniel H. / Medical Laboratory Observer Notes to editors About Oxford Gene Technology * Founded in 1995 by the pioneer of Southern Blotting, Professor Sir Edwin Southern, OGT operates out of Begbroke Business Park near Oxford, with excellent access to a growing network of life science companies * OGT offers a comprehensive custom microarray consultancy service, from experimental design through all stages to data analysis and interpretation. Oxford Gene Technology Grants Microarray License to Aushon BioSystems by Business Wire Electrophoresis equipment similar to that used for Southern blotting is required. Launching a DNA lab by Farkas, Daniel H. / Medical Laboratory Observer			Southeast Asia Treaty Organization SEATO Southeast Asian art and architecture Southeast Asian arts Southeast Asian languages Southeast Drift Current Southeast Fairbanks Census Area Southeast Indian Southeast Islands Southeast, Institute of the Agriculture of the southeaster Southeastern Bureau of the Central Committee of the RCP B Southeastern Railroad Southeastern Union of Cossack Hosts, Mountaineers of the Caucasus, and Southend-on-Sea souther southerly southerly burster southern Southern 500 Southern Africa Southern African Heat Low Southern Alps southern bean mosaic virus southern bean mosaic virus group Southern Beech Southern blotting Southern Building Code Congress International Southern Bureau of the Central Committee of the RSDLP Southern California, University of Southern Carpathians Southern Christian Leadership Conference Southern Coast of the Crimea Southern Colonial house Southern Common Market Southern Cross Southern Crown Southern Economic Region Southern Executive Committee Southern Fish Southern Floristic Kingdom Southern Guinea Upland southern hemisphere Southern Ice Field Southern Illinois University Southern Indian Ocean Anticyclone Southern Kazakh Merino Southern Khangai Plateau southern lights Southern Luo Southern Methodist University Southern Morocco			Southern Bell Data Network Southern Bell Telephone and Telegraph Southern Belle Swing Bash Southern Berkshire Golden Retriever Club Southern Berkshire Regional School District Southern Berkshire Youth Coalition Southern Bicycle League Southern Billiards and Snooker Association Southern Biosystems Southern Black Widow Southern Black Widow Southern Blackmouth Cur Southern Blackmouth Cur Breeders Association Southern Bleached Softwood Southern Bleached Softwood Kraft Southern blot Southern blot Southern blot Southern blot analyses Southern blot analysis Southern blot analysis Southern Blot Hybridisation Southern blot hybridization Southern Blot Hybridization Analysis Southern blot technique Southern blot technique Southern blot technique Southern blot technique Southern blot test Southern blots Southern blotting Southern blottings southern blue flag Southern Blue Nile Southern Blue Ridge Province Southern blue whiting Southern Blue-ringed Octopus Southern bluefin tuna Southern Bluefin Tuna Management Advisory Committee southern bog lemming Southern Boobook Southern Boobook Southern Border Provinces Administration Centre Southern bottlenose whale Southern bottlenose whale Southern Boubou southern boubous Southern Branch of the University of California Southern Brazoria County Community Networking Southern British English Southern Broadcast Services, Inc. Southern Bronx River Watershed Alliance Southern Brown Kiwi Southern Brown Kiwi Southern Brown Kiwi Southern brown mamba Southern Buckeye Athletic and Academic Conference southern buckthorn Southern Bug Southern Bug Southern Bug

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