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Southern blotting |
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Southern blotting [′sə·th·ərn ‚bläd·iŋ] (cell and molecular biology) A technique for the detection of specific sequences among deoxyribonucleic acid (DNA) fragments whereby the fragments are separated by gel electrophoresis and then blotted onto a sheet of nitrocellulose for detection with radioactively labeled nucleic acid probes. It was first described by E. M. Southern in 1975. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Families of closely related genes are difficult to analyze using standard techniques--because of high sequence similarity, a closely related gene or pseudogene can easily be mistaken for the target gene during Southern blotting or PCR amplifications. After AluI digestion, the DNA was transferred to a nylon membrane for polymorphic guanine cytosine-rich repetitive sequence (PGRS) Southern blotting. This study demonstrates that considerable laboratory technician time could be saved by referring only non-normal outcomes for additional tests through PCR-acrylamide gels and Southern blotting. |
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