This mutation is located on chromosome arm 3L and has as a direct effect an increased number of nuclear divisions before cellularization of the embryo sac, which generates in the embryo sac an indeterminate, extra number of micropylar and synergids
cells, egg cells, central cells, and polar nuclei within central cells (Evans, 2007; Guo et al.
In this study, both synergid cells were seen in the embryo sac.
The mature embryo sac is composed of 7 cells, one central cell contained polar nuclei or secondary nucleus, two synergids and one egg cell that formed egg apparatus and three antipodal cells that are degenerate in the mature embryo sac before fertilization.
Based on present embryological studies, many exceptional events were reported in the members of this family, including Nemec phenomenon (Davis, 1968; Batygina, 1987), increasing of synergids (Cichan and Palser, 1982), increasing of antipodal cells (Richards, 1997; Pandey, 2001), four-celled female gametophyte (Harling, 1951) and apomixis (Davis, 1968; Chaudhury et al.