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Thiochrome

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Thiochrome 

(C12H14ON4S), a product of the oxidation of thiamine in an alkaline medium. Thiochrome crystals are yellow. Aqueous solutions in ultraviolet light (λmax = 460–470 nm) are intensely fluorescent. A highly sensitive method of quantitative determination of thiamine and thiamine pyrophosphate (cocarboxylase) used in medical and biological research is based on the determination of the specific fluorescence of thiochrome.
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Because of the low biological concentrations of these analytes, most methods employ precolumn or postcolumn oxidation of thiamines followed by fluorescent detection of thiochrome compounds.
Rapid HPLC measurement of thiamine and its phosphate esters in whole ... by Lu, Jun; Frank, Elizabeth L. / Clinical Chemistry
In October 1938, six months after I had joined Kraft, he asked me to use the recently published Jansen thiochrome method to determine the vitamin B 1 (thiamin) in Vegemite.
Australian food science 2 *: 1940 to 1970--some personal ... by Farrer, Keith / Nutrition & Dietetics: The Journal of the Dietitians Association of Australia
The chromatographic profile of a blank injection prepared by omitting the thiochrome reaction suggested a lack of interference in sample extracts (Fig.
Vitamin [B.sub.1] status assessed by direct measurement of thiamin ... by Talwar, Dinesh; Davidson, Helen; Cooney, Josephine; St. JO'Reilly, Denis / Clinical Chemistry
 
 
 
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