Thiochrome


Also found in: Medical.

Thiochrome

 

(C12H14ON4S), a product of the oxidation of thiamine in an alkaline medium. Thiochrome crystals are yellow. Aqueous solutions in ultraviolet light (λmax = 460–470 nm) are intensely fluorescent. A highly sensitive method of quantitative determination of thiamine and thiamine pyrophosphate (cocarboxylase) used in medical and biological research is based on the determination of the specific fluorescence of thiochrome.

References in periodicals archive ?
Precolumn derivatization of thiamine yields a fluorescent thiochrome that is isolated by isocratic elution on a reversed-phase HPLC column and detected by fluorescence.
To assess the stability of the thiochrome derivatives, controls and patients samples (n = 25) were assayed immediately after potassium ferricyanide oxidation, then stored at 10[degrees]C in the dark and reassayed after 24 and 48 h.
Concentrations of thiochrome products stored in the dark at 10[degrees]C decreased by no more than5%in 48 h.
In the early 1940s, RA Bottomley at Kimptons in Melbourne began to use the thiochrome method to study thiamin in flours and later measure riboflavin.
A reliable semi-automated method for the determination of total thiamin in whole blood by the thiochrome method with high performance liquid chromatography.