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Transphosphorylation

   Also found in: Medical 0.01 sec.
Transphosphorylation 

the enzymatic transfer of a phosphoric acid residue (phosphategroup, —PO32–) from one compound to another in living cells. Transphosphorylation links the most important reactions of metabolism in the cell, carrying out an exchange of energy between various processes by forming and breaking energy-rich phosphate bonds. In the majority of transphosphorylation reactions, the phosphate is transferred to the hydroxyl group of an alcohol or carbohydrate, with the formation of an energy-poor bond. A molecule of adenosine triphosphate (ATP) usually serves as the donor of the phosphategroup. The transphosphorylation reaction is catalyzed by phosphotransferase enzymes, which usually require the presence of Mg2+ to become active.



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Using site-directed mutagenesis, nine cysteine residues (675, 722, 748, 787, 866, 917, 961, 1094, and 1105), within JAK2 were individually and progressively mutated to serines and the effects of these mutations were tested in autophosphorylation and transphosphorylation assays.
Mutagenesis of a critical Lys882 residue to a glutamate residue abolished all evidence of kinase activity, confirming that the observed phosphorylation of Tyr-to-Phe mutants was not a transphosphorylation catalyzed by another kinase.
 
 
 
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