UUO operation did not significantly change total Smad 2/3.
UUO operation can lead to marked renal fibrosis in mice (Chevalier et al.
In addition, we found PF (50, 100 mg/kg) and losartan (50 mg/kg) could also inhibit PAI-1 expression induced by UUO.
In the present study, we found PF and losartan could inhibit the EMT during renal fibrosis in UUO mice through preserving E-cadherin expression and inhibiting excessive expression of [alpha]-SMA, further, PF (50, 100 mg/kg) and losartan (50 mg/kg) could inhibit Snail mRNA expression during UUO.
In this study, we found that TGF-[beta]1 mRNA expression was dramatically induced in UUO kidneys, and PF (100 mg/kg) and losartan (50 mg/kg) significantly reduced the expression.
Herein we found that the mRNA expression of BMP-7 after UUO decreased sharply to about half of that in Sham group, and PF (25, 50, 100 mg/kg) and losartan (50 mg/kg) markedly preserved the mRNA expression of BMP-7.
In this paper, we found that the ratio of the mRNA of TGF-[beta]1 and BMP-7 elevated disastrously after UUO, and PF down-regulated the ratio in a dose-dependent manner.
In this study, we investigated the activation of Smad signaling during UUO, and found PF (50, 100 mg/kg) significantly inhibit the phosphorylation of Smad2/3 induced by UUO.