Western blotting


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Western blotting

[′wes·tərn ‚bläd·iŋ]
(cell and molecular biology)
A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest.
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North America is the largest market for western blotting, followed by Europe and Asia.
The total cell lysates (20 [micro]g of proteins) were analyzed by Western blotting for bax, bad, bcl-xS/L, and bcl-2 proteins (Figure 1A-D).
With manufacturing facilities in Pennsylvania, Rockland produces Antibodies for western blotting, immunohistochemistry (IHC), immunofluorescence microscopy, ELISA and FLOW cytometry.
ProteinSimple offers high-throughput Western blotting instruments which boosts lab productivity.
Western Blotting is a common technique in life science research, including detection of proteins associated with diseases.
In-Cell Westerns are performed on fixed cultured cells in 96- and 384-well microplates, and bypass the gels and cell lysates typically required for Western blotting.
apparatus for electrophoresis and transfer of proteins to the membrane, which will be used in particular to monitor the expression of proteins of interest using sds-page and western blotting.
Western blotting procedures using unheated antigens were performed (4).
ATLANTA -- Serologicals Corporation (NASDAQ: SERO) today announced that Upstate Group, its wholly owned subsidiary, has released a new, more cost efficient Western blotting detection kit based on its high quality Visualizer(TM) Western Blot Detection Kit.
This proposed contract is for the supply, installation and commissioning associated with the assitance to the use of technical equipment for western blotting.
The quantum dot-antibody conjugates generated with this kit can be used in a broad range of applications including: immunohistochemistry, tissue staining, plate-based immunoassays, western blotting, flow cytometry and other user-designed experiments.