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Western blotting |
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Western blotting [′wes·tərn ‚bläd·iŋ] (cell and molecular biology) A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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In addition, we used reverse-transcriptase polymerase chain reaction and Western blotting to demonstrate significantly increased levels of the apoptotic effecter caspase-3 in treated animal hearts. Regarding the mechanism of action of Defibrotide, initial Western blotting results show that Defibrotide reduces phosphorylation-activation of p70S6 kinase, which is a key target in the PI3K/Akt/mTOR signaling pathway linked to angiogenesis. Serological differentiation of murine typhus and epidemic typhus using cross-adsorption and western blotting. |
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