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Yvelines |
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Yvelines (ēvlēn`), department (1990 pop. 1,320,000), N central France, W of Paris. Versailles Versailles (vərsī`, Fr. vĕrsī`), city (1990 pop. 91,029), capital of Yvelines dept., N central France. ..... Click the link for more information. is the capital. |
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5% agarose gel electrophoresis were visualized by ethidium bromide staining, purified by using MultiScreen-PCR Filter Plate (Millipore, Saint-Quentin en Yvelines, France), and sequenced in both directions by using the d-Rhodamine Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer, Coignieres, France). DNA was extracted by using Fastprep DNA extraction kit (Ozyme, St Quentin en Yvelines, France) according to the manufacturer's recommendations and was subjected to polymerase chain reaction (PCR) targeting the 16S rRNA gene as previously described (2). The reaction products were resolved on 5% polyacrylamide gels (Tebu, Le Perray en Yvelines, France) by an ABI PRISM 377 DNA sequencer (PE Applied Biosystems). |
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Yvelines
