ADP-RIBOSYLATION OF EEF2 IN A COLORIMETRIC SOLID-PHASE ASSAY
To compare ADP-ribosylation of soluble eEF2, as described above, with immobilized eEF2,1.2 [micro]g eEF2 in PBS was incubated for 2 h directly after precoating with anti-his antibody and then washed to remove unbound eEF2.
DETECTION OF ADP-RIBOSYLATION AFTER WESTERN BLOTTING
The enzymatic activity of both chimeric toxins was demonstrated by ADP-ribosylation with 420 [micro]g/L protein and detection by Western blotting (Fig.
ADP-ribosylation factor 6 regulates mammalian myoblast fusion through phospholipase D1 and phosphatidylinositol 4,5-bisphosphate signaling pathways.
Endocytosis of CT may follow one of three pathways: (i) lipid raft/caveolae mediated endocytic pathway, (ii) clathrin mediated endocytic pathway, or (iii) ADP-ribosylation factor 6 (Arf6)-associated endocytic pathway.
Partial blocking of the ADP-ribosylation site can be achieved by adding peptides to the amino terminal of CTA1 that produces steric hindrance on the enzymatically active site, thereby reducing the toxicity (56).
Further, tryptophan and niacin are converted to nicotinamide adenine dinucleotide, which is a substrate for poly (ADP-ribosylation) of histones and other DNA-binding proteins.
Increase in histone poly (ADP-ribosylation) in mitogen-activated lymphoid cells.
DNA strand breaks alter histone ADP-ribosylation. Proc Natl Acad Sci USA 1989; 86 : 3499-503.