An improved strategy directed to strengthen the
Agrobacterium mediated transformation system is required to overcome barriers to transform monocots genetically.
Activation of bacterial strains:
Agrobacterium LBA4404-ElectroMAX[R] cells (transformed with bifunctional BinRD29A or pCAMBIA1301 plasmids) were cultured in 25 mL of liquid Luria-Bertani (LB) medium (10 g/L triptone, 5 g/L NaCl, 5 g/L yeast extract) containing the appropriate antibiotics and allowed to grow for 48 h at 28 [degrees]C with 160 rpm agitation according to Solis-Ramos, GonzalezEstrada, Nahuath-Dzib, Zapata-Rodriguez, & Castano (2009).
Advances in
Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.
Agrobacterium spp isolated from clinical specimens.
Sawada, "Classification and nomenclature of
Agrobacterium and Rhizobium," International Journal of Systematic and Evolutionary Microbiology, vol.
(2012) Traversing the cell:
Agrobacterium T-DNA's journey to the host genome.
Multiplex PCR assay for identification of commonly used disarmed
Agrobacterium tumefaciens strains.