DNases may be grouped into three functional categories: [Mg.sup.2+]--dependent endonucleases, [Ca.sup.2+]/[Mg.sup.2+]--dependent endo-nucleases (e.g.
Effect of aerosolized recombinant human
DNase on exacerbations of respiratory symptoms and on pulmonary function in patients with cystic fibrosis.
Reorganization of actin in platelets stimulated by thrombin as measured by the
DNase I inhibition assay.
The extension requires two additional steps to the traditional transformation protocol: to allow the students time to treat their DNA sample and then to have more plates available for the students to transform the appropriate number of aliquots of competent cells (usually six: no DNA negative control, positive control of plasma with no treatment, amylase, protease,
DNase, and RNase treatment).
In the same study, the researchers injected a chemical
DNase inhibitor into one hind leg of each of four mice infected with flesh-eating strep disease and injected a placebo into the other hind leg.
These were harvested, treated with
DNAse and the DNA released from the bacteria by boiling and freezing.
To further confirm that SYBR DX fluorescence on membranes was due to immobilized DNA or RNA, membranes were digested with
DNase or RNase.
has won marketing approval from the Food and Drug Administration for dornase alpha (a.k.a.,
DNase), which is a treatment that could extend the lives of thousands of patients with cystic fibrosis.
As it stands, the company hopes to bring three new products to market by mid-decade, including
DNase, a highly-touted treatment for cystic fibrosis.
On 7th July, culture yielded growth of CoNS that was characterized phenotypically as Staphylococcus haemolyticus, based on coagulase and
DNAse tests and biochemical reactions.