Encyclopedia

Ehrlich's reagent

Ehrlich's reagent

[′er·liks rē‚ā·jənt]
(organic chemistry)
(CH3)2NC6H4CHO Granular or leafletlike crystals that are soluble in many organic solvents; melting point is 74°C; used in the preparation of dyes, as a reagent for arsphenamine, anthranilic acid, antipyrine, indole, and skatole, and as a differentiating agent between true scarlet fever and serum eruptions.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive
Most methods rely on the reaction of porphobilinogen with Ehrlich's reagent, coupled with a purification step to remove interfering chromogens.
Rapid quantitative method using spin columns to measure porphobilinogen in urine
Actually, the SPF is first sorted into P and SF with FBBS reagent, and then the SF into F and hence S with Ehrlich's reagent. The SPF samples must be freshly cut and contain heartwood.
Chemical answers to the sorting of commercial woods in Canada
It is performed by mixing 1 mL of urine with 1 mL of p-dimethylaminobenzaldehyde in acid solution (Ehrlich's reagent).
Specificity and sensitivity of the method are increased by adsorbing the PBG on an ion-exchange resin in a column, washing off interfering compounds, eluting the PBG, and reacting it with Ehrlich's reagent (3).
The urine sample is adsorbed, washed, and eluted through a series of procedures involving the addition and removal of filters and final color formation with Ehrlich's reagent. A color chart and a dye color calibrator are provided for visual assessment.
Ehrlich's reagent (2 mL; 1 g of p-dimethylaminobenzaldehyde in 50 mL of 6 mol/L HCl) was added, the bottle was stoppered, and the contents were mixed rapidly by inversion.
Testing for porphobilinogen in urine
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