C-X-C Chemokine Receptor Type 2 (CDw128b or GRO/MGSA Receptor or High Affinity Interleukin 8 Receptor B or
IL8 Receptor Type 2 or CD182 or CXCR2) - CXCR2 or Interleukin 8 receptor, beta is a chemokine receptor.
(A) The gene expression of pro-inflammatory cytokines IL1B, IL6,
IL8, and TNF was measured by qPCR.
In the adipose CM, we found that interleukin-8 (
IL8), interleukin-6 (IL6), GRO1 oncogene (CXCL1), and fractalkine (CX3CL1) were present in high levels among several others (total 25 factors) detected above control SFM.
The supernatant was collected for quantitative analysis of proinflammatory cytokines (IL6,
IL8, IL10, and TNF-[alpha]) using an enzyme-linked immunosorbent assay kit with a specific antibody to each cytokine (Quantikine[R] Colorimetric Sandwich ELISA, R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.
These studies revealed numerous inflammatory molecules to be implicated in the PVR development, such as growth factors (PDGF, HGF, VEGF, and EGF), transforming growth factors (TGFA, TGFB), molecules from the SMAD family and interleukins (IL1, IL6,
IL8, and IL10), tumor necrosis factors (TNF), and tumor suppressor protein (p53) [10-15].
Herein, we assessed cytokines and growth factors crucial for eosinophil proliferation and differentiation (GM-CSF, IL5, and IL4), for their release from bone marrow (IL5), for their survival (IL5, IL13, GM-CSF, and IL4) and priming in circulation (IL5, GM-CSF, IL4, and TNF[alpha]), and for extravasation (IL4, IFN[gamma], TNF[alpha],
IL8, and eotaxin) and homing into the gut (eotaxin, RANTES, IL5,
IL8, IL13, GM-CSF, and TNF[alpha]) as well as responsible for inducing production and secretion of their mediators (TNF[alpha], IFN[gamma], IL-5, GM-CSF, and eotaxin) (reviewed in [10, 17, 20]).
We evaluated the mRNA expression of the following proinflammatory cytokine [interleukin-1a (IL1A), interleukin-6 (IL6), interleukin-8 (
IL8) and tumour necrosis factor (TNF)] and bone formation [alkaline phosphatase (ALP), collagen type 1 (COL1) and runt-related transcription factor 2 (RUNX2)] genes.
Khayani was conferred PhD degree on his original study with the title 'A Clinico Pathological co relative Study on Oral Pre-Neoplasia and Oral Cancer with detection of early bio-markers HPV-16, and 18, IL6 and
IL8 in Saliva.'
Association of duffy blood group gene polymorphisms with
IL8 gene in chronic periodontitis.