Escherichia coli were identified based on growth MacConkey agar followed by gram staining and biochemical testing using catalase, oxidase,
IMVIC test. Further classification of isolated Gram negative strains was performed using Microbacter GNB 24E (Oxiod, UK).
Klebsiella species were identified by their characteristic growth (lactose positive, dome-shaped, mucoid and sticky colonies on MacConkey agar (Oxoid) and by biochemical tests (
IMViC test).
IMViC Test. The production of tryptophanase, sufficient amount of alcohol, acetyl methyl carbinol, and citrate utilisation were noted using the indole test, methyl red (MR) test, Voges-Proskauer (VP) test, and citrate utilization test, respectively, according to the protocol of Mackie McCartney [14].
The cultures giving + + - - reaction for
IMViC test were confirmed as E.
All isolates showed identical reaction to
IMViC tests i.e -+-+.