Previous research has demonstrated that ACE inhibitory peptides have some common characteristics: i) The C-terminal tripeptide sequence strongly affects binding to ACE even though the underlying mechanism has yet to be completely established [20]; ii) C-terminal hydrophobic amino acids can effectively bind to the ACE active site [21]; iii) Di- or tripeptides with aromatic or branched-chain amino acids at the
N-terminus and Tyr, Phe, Trp, or Pro at the C-terminus have interactions with ACE active site amino acid residues due to their strong affinity towards the ACE active site [7,22-24].
Identified antioxidative peptides of casein range from 2-14 amino acids in length containing hydrophobic amino acids at the
N-terminus and/or C-terminus and proline, histidine, or tyrosine within the sequence (Li and Li.
pET28a was used as expression vector, so that the expressed CueO will have a His-tag on its
N-terminus. The so far cloned construct pTZ57-cueO as well as pET28a (expression vector) were digested with NdeI and EcoRI in order to produce complementary sticky ends.
Nesprin-1 and Nesprin-2 harbor at their
N-terminus paired calponin homology domains that mediate the binding to Factin [3, 4].
Though not a mutation in the calcium-binding EFh of the STIM1 protein, this mutation is thought to destabilize the ncEFh/cEFh-SAM interaction, converting the
N-terminus of STIM into an active conformation, regardless of calcium binding [5].
However, the role of PB1-F2
N-terminus is poorly understood, although two short and weak a-helixes were proposed [17].
UB-311 is a novel UBITh active vaccine targeting the
N-terminus of A?, designed to induce high B-cell specific responses while avoiding T-cell inflammation.
Pairwise alignment reveals that pneumolysin do not have the
N-terminus signal peptide sequence which is present in the template i.e.
One of these antibodies is always specific for the intact cysteine ring, which is thought to be the active form, while the other antibody recognizes either the C-terminus of the peptide (Abbott AxSYM and Architect, Shionogi IRMA) or for the
N-terminus (Alere Triage and Beckman Access).