pure culture

[′pyu̇r ¦kəl·chər]

(microbiology)

A culture that contains cells of one kind, all progeny of a single cell.

The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Pure Culture

a culture containing cells of a single species of microorganism.

The most reliable way of isolating a pure culture is from a single cell of a population. For yeast and microscopic fungi, this is accomplished by Lindner’s drop technique. Drops from a cellular suspension of yeast or fungi spores (conidia) are transferred to a cover glass, or cover slip. The cover glass is then placed over a cavity slide. Microscopic techniques are used to find a drop containing a single cell, and the drop is then transferred, using sterile procedures, to a culture medium. Pure, single-cell bacterial cultures are isolated with the aid of a micromanipulator or thin capillaries (B. V. Perfil’ev’s microselector).

The isolation of pure cultures is necessary in the classification and study of the variability of microorganisms and in the production of vaccines. It is also used in the microbiological industry for the preparation of enzymes, antibiotics, vitamins, steroid hormones, and other preparations.

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References in periodicals archive

The isolation of pure cultures of lactic acid bacteria was done according to the conventional method.

The characteristics of lactic acid bacteria isolated in North Ossetia-Alania

One hundred pure culture plates were prepared from the original culture plates from each organ.

FUNGAL INFECTION IN SILVER CARP, HYPOPHTHALMICHTHYS MOLITRIX (VALENCEINNES) REARED IN EARTHEN POND

These pure cultures are considered as first generation culture (mother cultures) and hyphal tips were inoculated to obtain the second-generation culture.

Rohitukine, a chromone alkaloid and a precursor of flavopiridol, is produced by endophytic fungi isolated from Dysoxylum binectariferum Hook.f and Amoora rohituka (Roxb).Wight & Arn

The same trend was evident in the presence of both sanitizers indicating that the addition of sanitizers to the medium did not influence the toxicity of O113 in pure culture. Growth of E.

Influence of sanitizers on the lipopolysaccharide toxicity of Escherichia coli strains cultivated in the presence of Zygosaccharomyces bailii

Yeast-like organisms were isolated from urine samples on three consecutive days as pure cultures. It was identified as Trichosporon asahii using API ID 32C.

A rare case of urinary tract infection due to Trichosporon asahii

This proves the possibility of using a pure culture of Staphylococcus xylosus in dulce de leche, aiming at helping with development of special aroma, taste and texture to such products.

Viability of Staphylococcus xylosus during shelf-life of dulce de leche prepared by vacuum evaporation/Viabilidade de Staphylococcus xylosus na vida de prateleira de doce de leite elaborado em evaporador a vacuo

typhimurium in pure culture is high, when in the presence of other microorganisms recovery may vary, the highest growth values were obtained in RP -this broth was productive and selective for S.

Estandarizacion de un metodo de recuento para Salmonella spp. y Shigella spp. en medios de cultivo liquidos especializados

Brad and I believed that we were the first to induce a malolactic fermentation from a pure culture. During preparation of our manuscript, we learned that Peynaud and Domecq had also been successful.

Missing collaborator

Ka3 150/150 (100%) Pure culture Uncultured AOB 150/150 (100%) Meadow soil, Osterfelder Kopf Germany Nitrosospira sp.

Does atmospheric C[O.sub.2] concentration influence soil nitrifying bacteria and their activity?

It's pure culture shock: Yu Shui is thrown into a unfamiliar mix of corporate work ethic, middle-class customers and a managerial attitude that immediately gives all employees English names--Yu Shui becomes "Cindy"; her co-worker Chen Bo Yu is "Jerry."

Up the Yangtze!

Starting with a pure culture or single colonies of the grown organisms, species identification is usually accomplished by a comprehensive determination of phenotypic profiles including Gram stain results, morphology, growth requirements, biochemical or biophysical properties, and when available, specific antigen or agglutination tests.

Melting of the ribosomal RNA gene reveals bacterial species identity: a step toward a new rapid test in clinical microbiology