The maps of every isolate were obtained for
restriction fragments in the range of 100-250 kb and were used to assess strain similarities.
Different molecular methods for the identification of rarely isolated nontuberculous mycobacteria and description of new hsp65
restriction fragment length polymorphism patterns.
The
restriction fragment patterns of these juveniles were identical to those of previously observed adult specimens, except for the two S.
Restriction enzymes seek a specific sequence, typically between four and eight bases in length, and cut the DNA at this location, creating discrete
restriction fragments. Scientists can harness this phenomenon for genomic research.
A large-scale DNA isolation procedure (Dvorak et al., 1988) was used for the
restriction fragment length polymorphism analysis (RFLP) and to optimize the amplification conditions for PCR markers.
Instead,
restriction fragments of the putative hybrid nuclear PCR product were always the same size as those of the sea urchin.
Restriction fragments were separated by gel electrophoresis through 2 per cent TBE agarose gel (Genei, Bangalore).
The significant differences obtained in dynamic range did not depend on which spectral band was chosen for quantification among the 10
restriction fragments, except for a pair of 7mers coming from the H3 motif.
For the restriction digestion, 7.5 to 10 [micro]L of PCR products were used on the basis of expected numbers and sizes of
restriction fragments.
Restriction fragments were separated on 2.5% (w/v) agarose gels containing ethidium bromide, and were photographed under the Gel Doc 2000 (Bio-Rad, Hercules, CA).
Restriction fragments length polymorphism analysis of Cryptococcus neoformans isolates from environmental (pigeons excreta) and clinical sources in New York City.